The den mother of the Shinsengumi Ayumu most often known as Ayunee or big sister Ayu is the cook and housekeeper at the Shinsengumi compound. A pretty woman with a motherly nature Ayumu is fully cognizant of what the people around her do for a living and acts as a sort of bigsister figure to Tetsu. She is Susumus older sister and like him a shinobi. She mentions in one episode them as being apart of the Oniwaban. While Susumu is based on the actual spy Ayumu is a fictional character.